Haematology of Cirrhinus mrigala fed with Vitamin C supplemented diet and post challenged by Aphanomyces invadens.

Disease outbreak in fish culture system interferes with productivity. Oral Immunostimulant containing vitamin C can result in activating the immune system in a nonspecific way thus providing resistance against pathogens. In the present study the fish Cirrhinus mrigala was fed with a feed supplemented with vitamin C (100mg/100g) for 40 days and post challenged with two different dilutions (102 and 105) of Aphanomyces invadans. The haematological parameters like TEC, TLC and differential leukocyte counts were analyzed 24hours, 72 hours and 7th day after infection. TEC counts and lymphocyte counts of infected fishes previously fed with control diet decreased significantly (43%, 36%) whereas it was minimal in fishes (21%, 8%) fed with Vitamin C supplemented diet. Post challenged fishes exhibited an increase in TLC in Vitamin supplemented diet (20%, 32%) over control diet fed fishes. In differential leucocyte counts the lymphocytes decreased in both the experimental groups (9%, 11%) whereas neutrophils increased in both, however the increase was higher in control diet fed group (34%). The basophils and eosinophils increased significantly in Vitamin C supplemented diet only (10%, 60%). Thus supplementation of feed with vitamin C enhanced fish’s tolerance to infection.

Immune response of catla catla fed with an oral immunostimulant plumbago rosea and postchallenged with aeromonas hydrophila.

The present study was designed to evaluate the immunostimulant potential of Plumbago rosea in fish catla, post challenged with Aeromonas hydrophila. Fishes were divided into 2 groups one received control diet and another received Plumbago rosea incorporated diet for 14 days. The haematological parameters and serum protein were estimated between control and experiment. There is An increase in TEC (P<0.05) and TLC (P<0.05) in the immunostimulant administered (IS)diet was observed. Increase in lymphocyte count was noted in immunostimulant incorporated diet. Remarkable increase in Hb from 6.2 to 8g% and serum protein level from 0.4to 0.5g% was observed in experimental fishes The immunostimulant administered Catla catla when challenged with Aeromonas hydrophila showed a decrease in TEC and an increase in TLC. Increase in lymphocytes was also noted.

Studies on the immouostimulant activity of Coriandrum sativum and resistance to Aeromonas hydrophila in Catla catla.

The present study was designed to evaluate the immunostimulant potential of Coriandrum sativum is fish Catla catla, post challenged with A.hydrophila. Fish were divided into 2 groups one received control diet, another received Coriandrum Sativum incorporated diet for 14 days. The haematological parameters and serum protein were analyzed between control and experiment. There was a significant increase in TEC (P<0.01) and TLC (P <0.01) in the immunostimulant (IS) administered diet. Remarkable increase in Hb content from as 6.9.to 7.39% and serum protein level from 0.56 to 0.58g% was noted. The immunostimulant administered post challenged Catla catla showed a decrease in TEC at 103 cfu/ml and a drastic increase in TLC(<0.01) in both 103 and 105 cfu/ml Hb content on IS incorporated post challenged Catla catla showed an increase from 8.7 – 11.5g%.